A simple non-isotopic method for DNA fingerprinting with M13 phage

A simple non-isotopic method for DNA fingerprinting with M13 phage.

Recently it was discovered that the bacteriophage M13 contains in its protein III gene, tandem repeats of a 15-bp motif that can recognize a family of hypervariable minisatellites in vertebrate DNA (1). Southern blots of human DNA digested with Hae III and probed with 32p_iaDeled M13 revealed complex individual-specific DNA fingerprints, similar to those described by Jeffreys et al (2) with pro...

Improved method for the production of M13 phage and single-stranded DNA for DNA sequencing.

An improved method is described for the efficient production of M13 phage and M13 single-stranded (ss)DNA in a relatively short time period. Infection of E. coli (F') cells with as few as 5 phage particles can yield 10(12) phage particles/mL in 3 hours if the cells are grown in LB broth or SOB broth supplemented with about 5 mM Mg2+. The method tolerates large variations in the initial multipli...

DNA fingerprinting is any method for

A Simple and Rapid Leaf Genomic DNA Extraction Method for Polymerase Chain Reaction Analysis

In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...

a new type-ii fuzzy logic based controller for non-linear dynamical systems with application to 3-psp parallel robot

abstract type-ii fuzzy logic has shown its superiority over traditional fuzzy logic when dealing with uncertainty. type-ii fuzzy logic controllers are however newer and more promising approaches that have been recently applied to various fields due to their significant contribution especially when the noise (as an important instance of uncertainty) emerges. during the design of type- i fuz...